ix83 inverted fluorescence microscope frame  (Evident Corporation)

Journal: Genetics

Article Title: Automated cell lineage reconstruction using label-free 4D microscopy

doi: 10.1093/genetics/iyae135

Figure Lengend Snippet: a) Generalizability of embGAN model across multiple source microscopes. Images of early and mid-stage N2 embryos shown in DIC, embGAN output, and merged channels acquired on the same microscope as the embGAN training data (Olympus IX83) and b) a second microscope using different hardware (Leica DM6000).

Article Snippet: Imaging was performed using either DIC alone for N2 or both DIC and fluorescence for JIM113 using an Olympus IX83 inverted frame equipped with a UPLSAPO60xs2 objective, a Visitech iSIM multipoint confocal scanner, ASI MX2000XYZ stage, and Hamamatsu Orca Fusion camera.

Techniques: Microscopy

Journal: bioRxiv

Article Title: Automated Cell Lineage Reconstruction using Label-Free 4D Microscopy

doi: 10.1101/2024.01.20.576449

Figure Lengend Snippet: A) 3D rendering of amphid cell positions traced using embGAN images B) Image of DIC and embGAN (red) image with annotations showing positions of amphid cells. Cells present at the focal plane are circled with dots showing the XY position of cells present at different depths in the volume.C) Accuracy of lineaging cells born late in embryogenesis. Mean (blue line) and per-embryo (red circles) error rates for lineaging amphid neurons in embGAN processed images. D) Generalizability of embGAN model across multiple source microscopes. Images of early and mid-stage N2 embryos shown in DIC, embGAN output, and merged channels acquired on the same microscope as the embGAN training data (Olympus IX83) and a second microscope using different hardware (Leica DM6000). E) Heatmap showing the pair-wise branch distance comparing lineage-aligned cell cycle lengths between all N2 and JIM113 embryos. The single N2 embryo imaged using the 2nd microscope is highlighted at the boundary between N2 and JIM113 embryos. F) Distribution of pairwise branch distances between specific lineages within N2 (blue) and JIM113 (orange) embryos. Vertical black line shows the interquartile range (the 25th to the 75th percentile). *** denotes p<0.001 and ** denotes p<0.01 by the rank sum test.

Article Snippet: Imaging was performed using either DIC alone for N2 or both DIC and fluorescence for JIM113 using an Olympus IX83 inverted frame equipped with a UPLSAPO60xs2 objective, a Visitech iSIM multipoint confocal scanner, ASI MX2000XYZ stage, and Hamamatsu Orca Fusion camera.

Techniques: Microscopy